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Native human IGF-1 is a 70-amino-acid single-chain polypeptide that activates the IGF-1 receptor (IGF-1R), a receptor tyrosine kinase, driving downstream PI3K/Akt and MAPK/ERK signaling linked to cell growth and protein synthesis

The EMA classifies it as safe, and adverse effects reported in clinical trials are generally mild and transient

The intracellular sequestration and the paucity of free extracellular haem-iron ensures that there is scarce availability of this essential nutrient for pathogens ( Figure 1 Iron levels are suspected to be low in the upper respiratory tract, with an estimated daily exposure of approximately 10-25 g or around 1/1000th of that encountered by the gastrointestinal tract (Cloonan et al., 2017)

IIRI surgery Mice were weighed and anesthetized with 50 mg/kg pentobarbital sodium salt (Merck, Tc-P8411) after a 12-h fast